mcf10a cell lines (ATCC)

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ATCC mcf10a cell lines
Mcf10a Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 8141 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mcf+10a+cells/MCF+10A/pm42235302-221-12-19
Average 99 stars, based on 8141 article reviews

mcf10a cell lines - by Bioz Stars, 2026-10

99/100 stars

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Cell Culture:

Article Title: Curcumin Enhances Gemcitabine Sensitivity in Breast Cancer Cells Through ROS-Associated Mitochondrial Apoptosis and Transcriptional Reprogramming.
Article Snippet: Cells were cultured in Dulbecco’s modified Eagle medium (DMEM, high glucose formulation; Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific, Waltham, MA, USA), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). .. MCF-10A cells were cultured according to ATCC recommendations in Dulbecco’s modified Eagle medium/Ham’s F-12 (DMEM/F-12; Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 5% heat-inactivated horse serum, 20 ng/mL epidermal growth factor, 10 μg/mL insulin, 0.5 μg/mL hydrocortisone, and 100 ng/mL cholera toxin, in addition to 100 U/mL penicillin and 100 μg/mL streptomycin. ..

Article Title: Regulatory BC200 RNA promotes breast carcinogenesis by repressing BRCA1 gene expression
Article Snippet: .. MCF-10A cells and MCF-7 cells were purchased from ATCC and cultured following the manufacturer’s guidelines. ..

Article Title: TEAD1 condensates are transcriptionally inactive storage sites on the pericentromeric heterochromatin in cancer cells.
Article Snippet: MCF-7 cells were cultured in EMEM (ATCC, 30-2003) supplemented with 10% FBS and 10 μg ml−1 insulin, and MDA-MB-231 cells were cultured in DMEM (ATCC, 30-2002) supplemented with 10% FBS. .. MCF-10A cells were cultured in DMEM-F12 (ATCC, 30-2006) supplemented with 5% horse serum (Millipore, H1138), 20 ng ml−1 EGF (Rockland, 009-001-C26), 0.5 mg ml−1 hydrocortisone (VWR, 1315004), 100 ng ml−1 cholera toxin (Millipore, 9012-63-9), 10 μg ml−1 insulin and 1% penicillin–streptomycin (VWR, 10220-718). ..

Article Title: Curcumin Enhances Gemcitabine Sensitivity in Breast Cancer Cells Through ROS-Associated Mitochondrial Apoptosis and Transcriptional Reprogramming
Article Snippet: Cells were cultured in Dulbecco’s modified Eagle medium (DMEM, high glucose formulation; Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific, Waltham, MA, USA), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). .. MCF-10A cells were cultured according to ATCC recommendations in Dulbecco’s modified Eagle medium/Ham’s F-12 (DMEM/F-12; Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 5% heat-inactivated horse serum, 20 ng/mL epidermal growth factor, 10 μg/mL insulin, 0.5 μg/mL hydrocortisone, and 100 ng/mL cholera toxin, in addition to 100 U/mL penicillin and 100 μg/mL streptomycin. ..

Modification:

Multiple Displacement Amplification:

Article Title: Lipid Transfer Proteins and PI4KIIα Initiate Nuclear p53-Phosphoinositide Signaling.
Article Snippet: .. A549, BT-549, Cal33, HCT116, HS578T, MDA-MB-231, SUM159, SUM1315, HEK293FT, and MCF-10A cells were purchased from ATCC. .. MCF10A cells were cultured in DMEM/F12 (#11330-032, Invitrogen) supplemented with 10% fetal bovine serum (#SH30910.03, Hyclone), 1% penicillin/streptomycin (#15140-122, Gibco), 20 ng/mL EGF (#CC-4107, Lonza), 0.5 mg/mL Hydrocortisone (#H4001, Sigma), 100 ng/mL Cholera toxin (#C-8052, Sigma), and 10 μg/mL Insulin (#I-1882, Sigma).

Recombinant:

Article Title: Acute degron-mediated RUNX1 loss reprograms enhancer activity to epigenetically drive epithelial destabilization and initiate cancer hallmarks
Article Snippet: .. MCF-10A cells were purchased from ATCC and grown in DMEM:F12 (Hyclone: SH30271, Thermo Fisher Scientific, Waltham, MA, USA) with 5% v/v horse serum (Gibco: 16050, Thermo Fisher Scientific) + 10 μg/ml human insulin (Sigma Aldrich, St. Louis, MO, USA: I-1882) + 20 ng/ml recombinant hEGF (Peprotech, Rocky Hill, NJ, USA: AF-100-15) + 100 ng/ml cholera toxin (Sigma Aldrich: C-8052) + 0.5 μg/ml hydrocortisone (Sigma Aldrich: H-0888) + 50 IU/ml penicillin/ 50 μg/ml streptomycin and 2 mM glutamine (Life Technologies, Carlsbad, CA, USA: 15140-122 and 25030-081, respectively). ..

Incubation:

Article Title: BMP9 promotes osteogenic differentiation of BMSCs via the lncRNA SNHG3/miR-619-5p axis in a breast cancer bone metastatic microenvironment.
Article Snippet: Bone morphogenetic protein 9 (BMP9), the most potent osteogenic member of the bone morphogenetic protein (BMP) family, is well-documented to potently induce the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs).. However, its role in regulating osteogenesis in the breast cancer (BC) bone microenvironment remains poorly understood.. By constructing BMP9-overexpressing MDA-MB-231 cell lines through adenovirus-mediated exogenous overexpression of BMP9 or adding BMP9 recombinant protein to the culture system, as well as establishing a nude mouse humerus injection model, we explored the effect and mechanism of BMP9 in the BC bone metastasis microenvironment.

Review